CV | Salvador Buse

CV

PhD in Bioengineering, Sep 2020 -

Working with Erik Winfree, researching pattern formation in biochemical neural networks
G1 courses: data analysis and Bayesian inference in Python, biomolecular computation, mathematical biology
G2 courses: theory of machine learning, networks of relations, probability theory and stochastic processes, physical cell biology, DNA-based neural networks

MSci in Natural Sciences (Systems Biology), Oct 2019 - Jul 2020

Thesis project: studying Syn61, a synthetic E. coli strain, with Jason Chin
Courses in mathematical modelling, biological networks, and synthetic and executable biology

BA in Natural Sciences (Chemistry) , Oct 2016 - Jul 2019

Courses in chemistry (organic, inorganic, theoretical, physical, and biological), biochemistry and molecular biology, and mathematics

Afnan Azizi, Anne Herrmann, Yinan Wan, Salvador Buse, Philipp J Keller, Raymond Goldstein, and William A Harris. (2020). “Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina”. eLife. DOI: 10.7554/eLife.58635. Open Access PDF.
Wesley Robertson, Louise Funke, Daniel de la Torre, Julius Fredens, Thomas Elliott, Martin Spinck, Yonka Christova, Daniele Cervettini, Franz Böge, Kim Liu, Salvador Buse, Sarah Maslen, George Salmond, Jason Chin. (2021). “Sense codon reassignment enables viral resistanceand encoded polymer synthesis”. Science. DOI: 10.1126/science.abg3029.

PhD Student, Erik Winfree Lab, Sep 2020 -

Researching pattern formation and morphogenesis in cellular automata and chemical reaction diffusion systems.

Master’s Thesis Student, Jason Chin Lab, Nov 2019 - Apr 2020

To enable bacteria to make proteins containing up to three unnatural amino acids, the Chin lab built ’Syn61’, an E. coli strain whose genome uses only 61 of 64 codons, and is the largest synthesised to date. This works by ’recoding’: synonymously replacing all instances of three codons. I found that Syn61 still contains some recoded codons, and and studied their implications for Syn61 and future recoded genomes.

Medical Research Council Summer Student, Jason Chin Lab, Jun 2019 - Sep 2019

In Syn61, recoded codons are ‘blank’: they do not encode natural amino acids, so can be assigned to unnatural amino acids. First, however, the tRNAs recognising recoded codons must be removed. I deleted those tRNAs and found the new Syn61 strain to be viable, but less fit.

Summer Research Intern, Jim Ferrell Lab, Jun 2018 - Aug 2018

I studied the role of an APC/C subunit in cell cycle regulation using two approaches: first, finding interaction partners of the subunit in Xenopus egg extracts, and second, identifying any altered phenotypes in cultured human cells with mutant versions of the subunit.

Summer Research Intern, Bill Harris Lab, Jul 2017 - Sep 2017

I used two‑photon microscopes to generate 3D time‑lapse movies of the retinas of developing zebrafish. I wrote code (in MATLAB) to extract data from those movies and analyse the motion of the cell nuclei, to study the role of nuclear migration in retinal development.

Co-President, Mar 2018 - Mar 2019

I arranged a weekly lecture series of 15 leading scientists, and co‑chaired a research internships event. I helped to renew our relationship with Oxford’s Science Society, and jointly arranged a formal dinner in Cambridge and a field trip to the London Natural History Museum. I did this together with my co-president, Charlene.

President, Mar 2017 - Mar 2018

I arranged a weekly lecture series of 17 pre‑eminent scientists, which featured Sir Paul Nurse & Dame Ottoline Leyser. I also organised a research internships event, which now occurs annually, and a symposium showcasing research at Trinity.